Alain Jacquet

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WORK ADDRESS


Chulalongkorn University,
Division of Allergy and Clinical Immunology,
Department of Medicine, Faculty of Medicine, Oor-Por-Ror Building, 10th floor, Room # 1010/5 1873 Rama IV Road, Pathumwan, Bangkok 10330, Thailand.
Phone : 02 2564579
Fax : 02 6523100
Born February 13, 1965, Ixelles, Belgium

EDUCATIONS


- 1986-1989 Ph. D. in Chemistry. Option Biochemistry Mention “Grande Distinction”. Université Libre de Bruxelles, Department of General Chemistry
- 1982-1986 Licence in chemistry. Option Biochemistry Mention “Grande Distinction”. A belgian licence degree is roughly equivalent to a master’s degree in chemistry Université Libre de Bruxelles.

APPOINTMENTS


From 1989 to march 2009 : Principal investigator of the Laboratory of Experimental Allergy (formerly Department of Applied Genetics). Associate Professor at ULB.Université Libre de Bruxelles Institut de Biologie et de Médecine Moléculaires Laboratoire d’Allergologie Expérimentale
Design of recombinant vaccines against infectious diseases and since 1999, against house dust mite allergy.
Since april 2009 : Associate Professor at Chulalongkorn University. Principal investigator and Group leader of the Recombinant Vaccine Unit of the Division of Allergy and Clinical Immunology (Prof. Kiat Ruxrungtham, M.D.). Design of recombinant vaccine against house dust mite allergy.

TEACHING AT CHULALONGKORN


Principle and Techniques in Molecular Biology- 2005707
- Recombinant protein expression and protein purification (6h)
- Animal Biotechnology (2h)
Cellular and Molecular Biology- 3700501
- From DNA to protein (3h)
- Immune system (3h)
Basic and Applied Immunology-2011703
- Th differentiation (2h)
- Allergic response and the new immunotherapeutic treatments (3h)
- Cancer Immunotherapy (3h)
Medical Immunology- 3004791
- Allergy and Immunotherapeutic treatments (2h) Advanced Immunology-3004741
- Immunomodulation of the allergic responses (3h)
Basic Research Methods in Cell Biology-3001743
- Protein study methods (4h)
Research Orientation in Medical Microbiology-2005706
- How to conduct a basic science research project ? (2h)
Research Skills in Biomedical Sciences and Biotechnology-3000751
- Protein separation and purification (5h)
- Cell line cultures (5h)
- Immunogenetics-3004329 (start in 2014)
- Concept of Allergy vaccine (4h)
- Molecular and Cellular Biotechnology—3000736 (30h)
- Protein Expression and Purification – 3000757 (30h)
- Protein Biochemistry- 3000763 (30h)
- Research Training Program in Biomedical Science (Meeting) Study Design/Methodology

WORKSHOP ORGANIZATION


Co-chairman and main instructor of the International Workshop on Protein Expression and Purification Strategies (Chulalongkorn University, Faculty of Medicine, Bangkok, Thailand, September 2010, October 2011, 2012 and 2013)

REVIEWER


Reviewer for International Archives of Allergy and Immunology, Allergy, Clinical and Experimental Allergy, Asian Pacific Journal of Allergy and Immunology

TECHNOLOGICAL EXPERTISE


Protein biochemistry (SDS-PAGE, Western blot, Glycosylation characterization, enzymatic digestion, protein assays, ….), Protein purification (any type of liquid chromatography including FPLC, ultrafiltration, precipitation, concentration, ….), Recombinant protein expression (in E.coli, yeast, insect and mammalian cells), animal cell culture (mammalian cell lines as well as primary immune cells), large scale cell culture in bioreactors, Recombinant DNA technology (cloning, PCR, sequencing), Immune assays : ELISA for antibody titer determinations, competition ELISA, Cytokine assays, T-cell reactivity, Dendritic cell activation, Basophil activation, Animal model of allergy, Airway hyperresponsiveness measurement, Bronchoalveolar lavage, Protein and naked DNA vaccination (formulation with adjuvants),…

INTERNATIONAL MEETINGS


More than 50 presentations

PUBLICATIONS


More than 55 publications in peer-reviewed journals